Re: pepsyn: ellman's test

Rod Levine (rlevine@nih.gov)
Mon, 12 Oct 1998 15:31:05 -0400

At 11:35 AM 10/12/1998 -0500, Wilhelm, Randy R wrote:
>I am new to the area of peptide synthesis and have seen mention of Ellman's
>reagent and Ellman's test to determine if disulfide formation is complete.
>I have just obtained Ellman's original paper (Arch. Biochem. Biophys. 82,
>70-77 (1959)). I am wondering how people actually use this.

Randy,

There have been a number of modifications introduced by various workers
since then. I'm sure there are many "recipes" in various cookbooks, but we
use this one:

Riddles, Blakely, and Zerner "Reassessment of Ellman's Reagent"
Methods in Enzymology 91:49 1983

Rod Levine

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