Iodination of peptides

Brett Phinney (phinney@bchserver.bch.ncsu.edu)
Tue, 13 Oct 1998 14:57:39 -0400

I was wondering if someone could give me some insight on the possible
iodination of tyrosine. I have been trying to iodinate my protein with cold
iodine. I have been using Pierce's iodogen coated tubes to iodinate my
protein. For a positive control I have been using Angiotension III
(RVYIHPF). The first and only time I tried this Angio III increased in mass
by 503.58 this seems to indicate 4 iodine's were added. Is this possible?
Were they all added to the tyrosine, or can they be added to other amino
acids as well?

Thanks a lot in advance

__________________________________
Brett Phinney
North Carolina State University
Department of Biochemistry
Ph# 919-515-5786, Fax 919-515-2047
ICQ# = 12442943