Re: Iodination of peptides

Michael N. Horst (horst_mn@Mercer.EDU)
Wed, 14 Oct 1998 08:13:58 -0400

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A small but important point, Seb- Iodogen ( and the bead version) is totally
different from chloramine T and allows iodination without polymerization of
the product ! Mike

Sebastian Joyce wrote:

> Dear Brett,
>
> Chloramine T (iodobeads) and lactoperoxidase catalyse oxidative iodination
> of histidine (present in angiotensin III) in addition to tyrosine. Both
> amino acids can be oxidised by two iodines each; this fits with the
> increase of four iodine mass to angiotensin III following iodination.
> Refer to page 3.3.2 of Colligan, et al. Current Protocols in Protein
> Science (John Wiley and Sons, NY) for the sites of iodination.
>
> Hope this helps.
>
> sebastian
>
> Sebastian Joyce, Ph.D.
> Assistant Professor
> Department of Microbiology & Immunology
> C6814, H107
> The Pennsylvania State University College of Medicine
> Milton S.Hershey Medical Center
> 500 University Drive
> Hershey, PA 17033-0850
>
> Telephone: 717-531-4163 (office)
> 717-531-4181 (laboratory)
> Facsimile: 717-531-4600

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A small but important point, Seb-  Iodogen ( and the bead version) is totally different from chloramine T and allows iodination without polymerization of the product ! Mike

Sebastian Joyce wrote:

Dear Brett,

Chloramine T (iodobeads) and lactoperoxidase catalyse oxidative iodination
of histidine (present in angiotensin III) in addition to tyrosine.  Both
amino acids can be oxidised by two iodines each; this fits with the
increase of four iodine mass to angiotensin III following iodination.
Refer to page 3.3.2 of Colligan, et al. Current Protocols in Protein
Science (John Wiley and Sons, NY) for the sites of iodination.

Hope this helps.

sebastian

Sebastian Joyce, Ph.D.
Assistant Professor
Department of Microbiology & Immunology
C6814, H107
The Pennsylvania State University College of Medicine
Milton S.Hershey Medical Center
500 University Drive
Hershey, PA 17033-0850

Telephone:  717-531-4163 (office)
            717-531-4181 (laboratory)
Facsimile:  717-531-4600

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