Human carbonic anhydrase 1

david andreu (andreu@admin.qo.ub.es)
Wed, 14 Oct 1998 19:25:14 +0100

Dear friends:
We are looking for carrier proteins having a single (and accessible)
Cys residue. A database search seems to suggest human carbonic
anhydrase 1 (HCA1) may be such a protein. The Sigma catalog (C4396,
purified , lyophilized; and C5290, partially synthesized from C4395,
about 2.5 times more expensive) does not specify this information,
which we haven't succeeded in obtaining from Sigma either. Can any of
the protein experts out there tell me whether the protein a) is
monomeric, and ii) the thiol group is available? As far as I've been
able to ascertain, the zinc atom in HCA1 is histidine, not
cysteine-bound. Thanks a lot for any help.

David

--
David Andreu
Scientific Director; Peptide Facility
University of Barcelona, Spain
Phone/Fax: 3493-402 1260
andreu@admin.qo.ub.es