Re: Suggestions for epitope selection software??

Marcus Macht (macht@sg17.chemie.uni-konstanz.de)
Thu, 15 Oct 1998 10:41:59 -0600

On Oct 14, 10:49am, Hao Xiao wrote:
> Subject: Re: Suggestions for epitope selection software??
> Sir,
>
> What exactly you mean that "however, there were also regions
> with high antigenicity in the protein used for immunization, which had
> not been covered by epitope regions but I had only four different
> mab-clones at hand so I have no idea if these regions did not result in
> an immunological response or if existing clones had not been selected
> for culturing". You mean " the antibodies resulted from immunized peptide
> fail recognize the protein or fail recognize the selected region of the
> protein?" could you provide more info.
>
> Thank

Hello!

What I meant was, that the immunization had been carried out with the intact
protein (approx. 300 aa) and after the immunization several clones had been
selected for culturing. All epitopes I found during the experiments had been
located in the middle part of the protein (aa 70-150). There are further
regions with a predicted high antigenicity e.g. in the C-terminal part of the
protein, but I don't now if there were no antibodies produced against these
regions or if they only had not been selected for culturing. To clarify this
question a much larger number of antibody clones would have to be investigated
to carry out a mapping of the entire "antigenic surface" of the protein.
So, in conclusion, the epitope regions identified by epitope mapping with
antibodies raised by immunization with the intact (!) 300 aa protein correlate
with predicted regions of high antigenicity in the protein.
I hope that this will answer your question.

Sincerely yours,
Marcus Macht

-- 
* Marcus Macht, AG Przybylski, Faculty of Chemistry, University of Konstanz
* Box M 732, 78457 Konstanz, Germany
* Tel:++49-7531-883391 / Fax:++49-7531-883097
* Email: macht@sgiclu.chemie.uni-konstanz.de
* URL: http://www.ag-przybylski.chemie.uni-konstanz.de/~macht