Xiaohui Chen
At 07:25 PM 10/14/98 +0100, you wrote:
>Dear friends:
>We are looking for carrier proteins having a single (and accessible)
>Cys residue. A database search seems to suggest human carbonic
>anhydrase 1 (HCA1) may be such a protein. The Sigma catalog (C4396,
>purified , lyophilized; and C5290, partially synthesized from C4395,
>about 2.5 times more expensive) does not specify this information,
>which we haven't succeeded in obtaining from Sigma either. Can any of
>the protein experts out there tell me whether the protein a) is
>monomeric, and ii) the thiol group is available? As far as I've been
>able to ascertain, the zinc atom in HCA1 is histidine, not
>cysteine-bound. Thanks a lot for any help.
>
>David
>
>--
>David Andreu
>Scientific Director; Peptide Facility
>University of Barcelona, Spain
>Phone/Fax: 3493-402 1260
>andreu@admin.qo.ub.es
>
>
>
Xiaohui Chen, Ph.D.
Department of Biochemistry
School of Medicine
Case Western Reserve University
2109 Adelbert Road
Cleveland, OH 44106
Ph: 216-368-3250
Fax: 216-368-3419
Email: xhchen@biochemistry.cwru.edu