Re: Phosphoserine/threonine MS/MS

Tony.Houthaeve (Tony.Houthaeve@rug.ac.be)
Wed, 21 Oct 1998 11:50:49 +0200 (MET DST)

>We have noticed that since installing delayed extraction on our MALDI-To=
f
>instrument, reflectron analyses of phosphoserine and phosphothreonine
>containing synthetic peptides give a low amount of the characteristic -9=
7=20
>dalton ion, with a more predominant peak at -88.3 to -88.9 daltons. Ha=
ve
>others seen this latter ion as well and does anyone have an explanation =
for
>its origin?
>
>Thanks,
>
>Kathy Stone/Ken Williams

There is also a nice paper on this topic discribed in:
Int. J. Mass Spectr. and Ion Processes=20
by Martina Schn=F6lzer & Wolf Lehmann in DEC 1997; 169 : 263-271.=20

This paper gives an overvieuw on this issue, also covering other modifica=
tion losses.

Tony H.

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Dr. Ing. Houthaeve Tony
VIB
Dept. Med. Prot. Research
University Gent
K.L. Ledeganckstraat 35
B-9000 Gent
Belgium

tel. (32) - 9 - 264 52 91
fax. (32) - 9 - 264 52 93

Tony.Houthaeve@RUG.ac.be
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