RE: Peptide synthesis

bcdchin@muccmail.missouri.edu
Wed, 21 Oct 98 14:58:00 CST

Bob,
You might try to make an activated ester like NHS of boc-gly-NHS (probable
commercially available) or gly-sulfo-NHS (sulfo-NHS activator avail. from
Pierce). React with your free peptide at pH 7-5 to 8. The alpha amino should
be mostly unprotonated while the ephison amino should be almost fully charged.
And then stop the reaction before your single lysine residue is modified.

No commercial endorsement is intended. Usual disclaimer applies.

David T. Chin
Director, Protein Core Facility
Univ. of Missouri - Columbia
Columbia, MO 65211

SHIPPING AND ACTUAL LOCATION:
2-17 Agriculture Building (office)
2-31 Agriculture Building (Lab)

e-Mail: chind@missouri.edu
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[mailto:chind@missouri.edu]
Phone: 573-882-2027
Fax: 573-882-7105

-----Original Message-----
From: Bao-Shiang Lee <boblee@uic.edu> at MU-Internet
Sent: Wednesday, October 21, 1998 2:39 PM
To: abrf@aecom.yu.edu at MU-Internet
Subject: Peptide synthesis

Hi everyone,

We are making a peptide G-HP-G-A-G-Q-PGKGRGPAPLQFGMAMMDMQDPGEV. Somehow we
miss the last G residue by mistake. Is there any way that I can couple G
to the rest of the peptide (already been cleaved and purified)? We always
can redo the synthesis. Thanks.

Bob Lee, UIC