DNASEQ: Big Dye and 373

mtholyst@smtpgate.bcsew.edu
Thu, 22 Oct 98 13:30:11 -0600

To 373 Sequencers:

We are interested in hearing from sequencing labs who are using Big
Dye chemistry with the 373 sequencer. Specifically, was the upgrade
worth the trouble? Are your reads significantly longer, is sample
prep comparable and can you reliably use the half strength dilution
and still get readable sequence?

We have read the ABRF sequencer study and have run test gels using a
377 instrument so we know the upgrade is possible. We would like to
know from users if it is practical as well.

Thanks in advance for your help,

Terri Fletcher
Trudy Holyst
Blood Research Institute
Blood Center of SE WI
Milwaukee WI

mtholyst@smtpgate.bcsew.edu