Linda-
I agree that the need for AAA is not as great as it used to be, but that there are times that it is invaluable. Perhaps the time is past for AAA methods that have dedicated instrumentation (e.g. 6300) or are instrument-specific. One reason we chose the Accutag chemistry is that it could be run on almost any HPLC system; the chemistry is manual, but the chromatography becomes just another technique where we only have to change the column and the buffers.
Vernon
Vernon A. Shoup
Regeneron Pharmaceuticals
Rensselaer, NY 12033
(518)488-6012
vernon.shoup@regpha.com
--------------------------------------
Date: 11/03/1998 12:14 PM
From: lsiconolfi-baez
Bryan,
Our 420 isn't "quite" mothballed. During the last three summers, when students (often high school or pre med) have asked to come into the lab, I've had them work on AAA. Within our institution, I've only had two single sample requests for AAA from researchers in those three years. I've denied them both because the machine was not "up and running". The time it would have taken to bring the system to a level at which I could produce the quality analysis they were used to getting from us in the past was prohibitive. As a result, we have decided to no longer provide AAA as a core lab service.
This is actually grievous to me, mostly perhaps because it follows a type of "market" driven philosophy which I so loathe. The request for protein quantitation may be small, but if the trend causes too many of us to shut down our operations, protein quantitation will fall into the realm of relativity.
Looking forward to hearing how others feel about the need AAA,
Linda
Big Apple Minute:
The sun is shining on this crisp election day. I will take it as encouragement that although the ballot of candidates again looks like making a choice between the lesser of evils, if I don't exercise my right to vote I'd be adding further lack of diligence to system falling apart from lack of use.
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Linda Siconolfi-Baez
Core Protein Sequencing Center
SUNY Health Science Cntr.
450 Clarkson Ave. Box 8 Brooklyn, N.Y. 11203
718-270-1267 (phone)
718-270-3316 (fax)
lsiconolfi-baez@netmail.hscbklyn.edu http://www.hscbklyn.edu/SUNY/Biochem/Bioparts/ProtSeq.html =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
______________________________ Reply Separator _________________________________
Subject: AAA Author: <bch143@abdn.ac.uk > at Internet
Date: 11/2/98 4:55 PM
Hello folks,
we are in the process of applying for funds to allow us to upgrade at
least one of our two 477's with a Procise. We also have a 420A AAA (no longer available) and we are wondering about the direction to go as regards this instrument. How many of you guys still feel a need for keeping your AAA's going?
I heard a rumour that there quite a few 420A's and other insruments mothballed out there. Grateful for any input.
Regards,
Bryan.
-- Bryan Dunbar
Protein Facility
Dept. of Molecular & Cell Biology
University of Aberdeen
Polwarth Building
Foresterhill
Aberdeen
AB25 2ZD
Tel:- +44 (0)1224 273103
Fax:- +44 (0)1224 273104
e-mail:- b.dunbar@abdn.ac.uk