Re: Mixed peptide sequencing

Frank_Masiarz@cc.chiron.com
Tue, 3 Nov 1998 13:45:56 -0800


Hi..............

Without some idea of the reason for your question, I can make a few
comments.

Please refer to:

Methods in Enzymology, 241, 302-310 (1994)

for a discussion of the use of synthetic peptide mixtures in the
determination of protease specificity.

However, in general, it is possible to sequence peptide mixtures with
a reasonable degree of certainty, if the relative signals differ by at
least a factor of two in initial yield. For example, components
present at 5, 10,20 and 40% of the total initial yield might reveal
unique sequences, depending on the residues present and the degree of
overlap. Interpretations are simplified if the sequence of the
protein and the mode of fragmentation which generated the peptide
mixture are known. It is possible to make "calls" which yield
successful oligonucleotide probes with unknowns. Needless to say, the
less complex the mixture, the better the results.

Good Luck !!

Frank R. Masiarz
Director
Protein Structure Analysis Group

Chiron Corporation


______________________________ Reply Separator _________________________________
Subject: Mixed peptide sequencing
Author: "Terry Stoming" <tstoming@MAIL.MCG.EDU> at SMTP
Date: 11/3/98 3:02 PM

Does anyone know anything about mixed peptide sequencing? How reliable is it?
Have you ever done it?