I am working with a mixture of dna and protein and need to hydrolyse both
consituents.
In the past I have used HF(room temp) or formic acid(60 degrees C) for DNA
hydrolysis and HCL(105 degrees C, 24 hrs) for protein hydrolysis. Will
HF/formic acid hydrolyse protein and will HCL hydrolyse DNA or what would
be an appropriate hydrolysis method?
Also, how does gas phase hydrolysis of these compounds compare to leaving
the compounds sit in the acid?
My review of the literature has not enabled me to answer these questions.
Any suggestions will be gratefully received.
Thanking you,
Kerryn Mason
Senior Research Fellow
Ray Williams Biomedical Mass Spectrometry Facility
UNSW
Sydney
Australia