From maria Lorenzi (Italy )

Maria Lorenzi (mlorenzi@ascu.unian.it)
Wed, 4 Nov 1998 13:01:15 +0100

Hi, I'm Maria.
I need help. Yesterday I load my toxin in the Sequencer to obtain the total
sequence of the monomer. We obtained it even if it is difficult to say if
the monomer has 50 residues or 51; in fact the background at the end is high
and we don't see an increment of any aminoacid.
Anyway, the 49 residue is a Tryptofan and in this sequencer there is the
problem that we cannot see it. I ask you why and in what way we can see it.
In this sequence we see, in that cycle, the increment of two peaks that
decrement in the subsequent cycle but we don't see the tryptofan. One of
these peaks is between the E and H while the other is immediately after the
alanine.
An other person had this problem with his sequence, he sequenced his
peptide, he got high sequence but in the corrispondence ot the tryptofan (
he previously knew that there was a tryptofan from the gene )he didn't see
anything but the increment of a peak closed the prolin.
It is strange that it could be a non proteic amino acid because the other
previous aminoacids are proteic.
Could you give an answer about these questions. In fact we want the total
and sure sequence of the toxin to clone the gene.
I thank you and I wait your anwser.
And please, could you suggest some procedures to see the tryptofan?
Ciao ciao
Maria