Re: hydrolysis protocols

Laurey Steinke (lsteinke@molbio.unmc.edu)
Wed, 4 Nov 1998 13:11:13 -0500

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Dear Richard,
We have obtained reproducible amino acid analysis results from samples with
large amounts of brown crud. (We use a Beckman 6300 with 110 degree HCl
(usually vapor) hydrolysis.) I am worried after reading your message.
Have we just been lucky? Have our results just been reproducibly low?

-laurey

Weather Incosequential: It has turned crisp and cold here. Most of the
leaves are off of the trees. My cub scout den is camping with a boy scout
troop again this weekend--luckily we have a heated cabin to sleep and eat
in, but it may be an awfully cold five mile hike Saturday!

Laurey Steinke
Protein Structure Core Facility
University of Nebraska Medical Center
Omaha Nebraska, 68198-4525

Phone (402) 559-6647
FAX (402) 559-6650
lsteinke@molbio.unmc.edu

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