Re: From maria Lorenzi ( Italy )

Laurey Steinke (lsteinke@molbio.unmc.edu)
Wed, 4 Nov 1998 13:43:07 -0500

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Angela c. Murphy: "Re: hydrolysis protocols"
Previous message: Michail Alterman: "Re: hydrolysis protocols"
In reply to: Maria Lorenzi: "From maria Lorenzi ( Italy )"
Next in thread: ricardo bastos cunha: "Re: From maria Lorenzi ( Italy )"

Dear Maria,
Sorry, I don't have a good answer. Tryptophan yeilds are low at best and
decrease dramatically as you get into the run. Could you digest the
peptide and separate the pieces, looking for absorbance at 280 to find the
part you wanted?

What sequencer are you running? Some of them you can tweak to reduce
background, but getting out 50 is really pretty good. Even with a reduced
background I don't thing you would be able to see tryptophan at cycle 49.

Good Luck,
-laurey

Weather Incosequential: Well, to those of you thinking I was quiet, here
I am in all my ususal verbosity. Just takes questions I know something
about you see.....

Laurey Steinke
Protein Structure Core Facility
University of Nebraska Medical Center
Omaha Nebraska, 68198-4525

Phone (402) 559-6647
FAX (402) 559-6650
lsteinke@molbio.unmc.edu

Next message: Angela c. Murphy: "Re: hydrolysis protocols"
Previous message: Michail Alterman: "Re: hydrolysis protocols"
In reply to: Maria Lorenzi: "From maria Lorenzi ( Italy )"
Next in thread: ricardo bastos cunha: "Re: From maria Lorenzi ( Italy )"