Re: Mixed peptide sequencing
Laurey Steinke (lsteinke@molbio.unmc.edu)
Wed, 4 Nov 1998 13:30:27 -0500
Dear Terry,
I don't know exactly what you mean--but if you mean do I ever read two
peptides off of one sequence run (like because there were two peptides in
the peak on the HPLC and one of them didn't fly in the Maldi)
yes--frequently. As Frank said, if the peptides differ in amount it can be
sorted through, although I must say that most such calls are tentative in
our lab because of the possibility of mixing up the peptides. When the
peptides are of the same amount it gets tricky. If there is any chance
that the peptides are in the data banks you can use protein prospector or
peptide pattern or findpatterns in gcg entering two amino acids at each
position and frequently come up with a match.
-laurey
Weather Inconsequential: Grey skies, maybe flurries tonight. Am I crazy to
celebrate my 41st birthday camping in weather like this? Maybe......
Laurey Steinke
Protein Structure Core Facility
University of Nebraska Medical Center
Omaha Nebraska, 68198-4525
Phone (402) 559-6647
FAX (402) 559-6650
lsteinke@molbio.unmc.edu