RE: AAA

Singleton, David H (david_h_singleton@groton.pfizer.com)
Thu, 5 Nov 1998 08:59:49 -0500

We too are in desperate need of a facility which will provide AAA in a
"timely" (3-4 days from receipt of sample) and "consistent" (There's nothing
in your sample, I don't know what could be the problem) manner. I've tried
university and commercial vendors for this and no one seems to "get it
right". We've taken to only assay peptide content by UV and HPLC
calibration based on volumes of sample. It seems like the world would beat
a path to your door if one provided a good AAA service.

-----Original Message-----
From: Howard, Eric F [mailto:eric.f.howard@vanderbilt.edu]
Sent: Wednesday, November 04, 1998 4:25 PM
To: Recipients of ABRF List
Cc: Recipients of ABRF List
Subject: Re: AAA

It is very interesting how people are not running AAA we have
consistantly run 200-300/year for the last 5 years. This has been a
very important part of our laboratory.

Eric

-----------------------------------------------------------------
Howard, Eric F
Vanderbilt University
Email: eric.f.howard@Vanderbilt.Edu
Protein Sequencing and Amino Acid Analysis Laboratory
615-322-6355

On Tue, 3 Nov 1998 15:35:10 -0500 (EST) Elizabeth Fowler
<efowler@ma.ultranet.com> wrote:

> I'm concerned to learn several labs are dropping AAA from the services
they
> offer. From the perspective of a small biotech company that needs AAA to
> characterize our products and quantitate the amount of protein in
> preparations, but needs it too infrequently to justify an in-house
machine,
> being able to send samples to high quality labs for analysis is important.
> I encourage those who still offer these services to let potential users
like
> me know who you are.
>
> Beth Fowler
>
> At 11:04 AM 11/3/98 -0500, lsiconolfi-baez@netmail.hscbklyn.edu wrote:
> >
> > Bryan,
> >
> > Our 420 isn't "quite" mothballed. During the last three summers,
when
> > students (often high school or pre med) have asked to come into the
> > lab, I've had them work on AAA.
> >
> > Within our institution, I've only had two single sample requests for

> > AAA from researchers in those three years. I've denied them both
> > because the machine was not "up and running". The time it would have

> > taken to bring the system to a level at which I could produce the
> > quality analysis they were used to getting from us in the past was
> > prohibitive. As a result, we have decided to no longer provide AAA
as
> > a core lab service.
> >
> > This is actually grievous to me, mostly perhaps because it follows a

> > type of "market" driven philosophy which I so loathe. The request
for
> > protein quantitation may be small, but if the trend causes too many
of
> > us to shut down our operations, protein quantitation will fall into
> > the realm of relativity.
> >
> > Looking forward to hearing how others feel about the need AAA,
> >
> > Linda
> >
> > Big Apple Minute:
> > The sun is shining on this crisp election day. I will take it as
> > encouragement that although the ballot of candidates again looks
like
> > making a choice between the lesser of evils, if I don't exercise my
> > right to vote I'd be adding further lack of diligence to system
> > falling apart from lack of use.
> >
> > =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
> > Linda Siconolfi-Baez
> > Core Protein Sequencing Center
> > SUNY Health Science Cntr.
> > 450 Clarkson Ave.
> > Box 8
> > Brooklyn, N.Y. 11203
> > 718-270-1267 (phone)
> > 718-270-3316 (fax)
> > lsiconolfi-baez@netmail.hscbklyn.edu
> > http://www.hscbklyn.edu/SUNY/Biochem/Bioparts/ProtSeq.html
> > =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
> >
> >
> >
> >
> >______________________________ Reply Separator
> _________________________________
> >Subject: AAA
> >Author: <bch143@abdn.ac.uk > at Internet
> >Date: 11/2/98 4:55 PM
> >
> >
> >Hello folks,
> > we are in the process of applying for funds to allow us to
> upgrade at
> >least one of our two 477's with a Procise. We also have a 420A AAA (no
longer
> >available) and we are wondering about the direction to go as regards
this
> >instrument. How many of you guys still feel a need for keeping your AAA's
> going?
> >I heard a rumour that there quite a few 420A's and other insruments
mothballed
> >out there.
> >Grateful for any input.
> >Regards,
> >Bryan.
> >--
> >Bryan Dunbar
> >Protein Facility
> >Dept. of Molecular & Cell Biology
> >University of Aberdeen
> >Polwarth Building
> >Foresterhill
> >Aberdeen
> >AB25 2ZD
> >Tel:- +44 (0)1224 273103
> >Fax:- +44 (0)1224 273104
> >e-mail:- b.dunbar@abdn.ac.uk
> >
> >
> >
> >
> >
> Elizabeth Fowler
> Director, Biochemistry and Process Development
> AutoImmune Inc.
> 128 Spring Street
> Lexington, MA 02421
> Phone: 781-860-0710 x 230
> FAX: 781-860-0705
> Email: efowler@ma.ultranet.com
>