Since there's been so much discussion of AAA recently, let me throw you a
small problem we've run into. We formulate and vial peptides for cancer
vaccine work and have the absolute concentration determined by quantitative
AAA by an outside contractor. We've recently had to formulate some
peptides in pure DMSO and our contractor is having trouble doing AAA in the
prescence of DMSO - they "failed to produce any reliable data" (their
words). This is presumably by gas phase hydrolysis, although I guess
there's plenty of DMSO still present as they would not be able to remove
easily it under vacuum.
Any hints at an alternative approach for the hydrolysis?
(must admit I didn't think it would create this much of a problem!)
Cheers,
Roger
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email murphy_r@licre.ludwig.edu.au