The answer is quite possibly, "yes." Many epitopes are only 6 residues
long and your polyclonal is probably recognizing several regions of the
23-mer. A monoclonal would be better, provided that it is raised against
or recognizes another part of the peptide.
Richard Laursen
---------------------------------
>Hi everyone,
>
>I have made a polyclonal antipeptide antibody against a peptide
>maeapq....RSC (23 a.a.). Can this antibody reconize another protein with
>maeapq...... (only 6 a.a. are the same)? If so, does monoclonal antibody
>helps. Thanks.
>
>
>Bob Lee, UIC
Richard A. Laursen
Department of Chemistry
Boston University
590 Commonwealth Ave.
Boston, MA 02215
Tel (617) 353-2491; FAX (617) 353-6466
email: <laursen@bu.edu>