Re: Cross reaction of a peptide antibody

Gottfried Proess (g.proess@eurogentec.be)
Mon, 09 Nov 1998 07:58:28 -0800

Bao-Shiang Lee wrote:
>
> Hi everyone,
>
> I have made a polyclonal antipeptide antibody against a peptide
> maeapq....RSC (23 a.a.). Can this antibody reconize another protein with
> maeapq...... (only 6 a.a. are the same)? If so, does monoclonal antibody
> helps. Thanks.
>
> Bob Lee, UIC

Bob,

following the answer of Richard, I suggest to purify by affinity your
polyclonal against a peptide of the same sequence but lacking the first
6 aa. In this way you should be able to separate the cross-reacting
antibodies and get out only the specific ones. This procedure would be
much less expensive and much less time consuming thant to restart the
whole procedure and make a monoclonal.
I hope this helps a little. 

-- 
Gottfried Proess
Tel.: + 32 4 366 01 50  Fax : + 32 4 365 16 04
E-Mail: g.proess@eurogentec.be
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