1998 ABRF DNA Sequencing Research Committee Study latest
George S. Grills (grills@aecom.yu.edu)
Tue, 10 Nov 1998 21:18:20 -0500
The latest results from the 1998 ABRF DNA Sequencing Research Commit=
tee
Study are now posted at
http://www.abrf.org/ABRF/ResearchCommittees/dsrcreports/abrfdna2/dsrc98.h=
tm.=20
The title of the study is =93Analysis of the Effects of Different DNA Seq=
uencing
Methods on Sequencing Quality, Creation of a Quality Control Resource, an=
d
Assessment of the Current State of the Art.=94 =20
The study examined the results of sequencing a standard template and
included 242 sequencing results from 72 machines in 59 laboratories. The =
first
goal of this study was to analyze the effect of a wide range of commonly =
used
sequencing methods and instrumentation on the quality of sequencing resul=
ts.
The effects of factors such as different machine types, dye chemistries,
reagent dilutions, and editing were examined. The second goal was to cre=
ate a
readily accessible web based resource of sequencing results. This resour=
ce can
be used as a benchmark for sequencing, as a reference for self-evaluation=
, and
for making decisions concerning new technologies. The third goal was to =
build
a current profile of DNA sequencing laboratories.=20
Preliminary results were presented in March 1998 at the ABRF annual
meeting and were posted on the web. A comprehensive analysis of the data=
was
presented in September 1998 at the 10th International Genome and Sequence
Analysis Conference. The details and an update of this latest analysis a=
re now
posted on the ABRF web site.=20
Please note that the ABRF DNA Sequencing Research Committee will con=
tinue
the 1998 study of different conditions on a standard template in 1999. T=
his
1999 study will include analysis of results using the latest new technolo=
gies
and chemistries. In addition, the DSRC is preparing challenging =91probl=
em=92
templates that will be the focus of a new 1999 study. The long term goal=
of
the new study will be to identify an array of templates that can be used =
as DNA
sequencing standards for evaluation and troubleshooting. Details of how =
to
participate in the 1999 studies will be posted in December 1998.