Hello everyone,
I am looking for the best way of accurately determining the
concentration of a peptide in the low microgram/ml range (say 2.5 - 25
microgram/ml = 1-10 micromolar). I am wondering if I could use a
spectroscopic or spectrofluorimetric technique (quantitative ninhydrin
test might do at > 5 micromolar; some kind of derivitization could maybe
be more sensitive). Recently I think TNBS or fluorescamine were
suggested in a similar context. Otherwise, I suppose I could label an
aliquot of the peptide during syntesis with biotin, and use an
enzyme-linked method in the quantitation. Any suggestions would be
really welcome. We want to measure peptide release from lipid vescicles,
so that the method should not interfere with this, and vice-versa. I
have no experience in this area, but am sure some ABRF members have.
Bye and thanks,
Alex
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Dr. Alessandro Tossi
Dept. of Biochemistry, Biophysics and Macromolecular Chemistry
University of Trieste
Phone -- 39 40 6763673; Fax -- 39 40 6763691
email: tossi@bbcm.univ.trieste.it
http://www.bbcm.univ.trieste.it/~tossi
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