autosampler help

Holt, John - PMC-US (JHolt@US.PMC-Vacc.Com)
Mon, 16 Nov 1998 19:11:48 -0500

I am hoping someone in the group can straighten out a newish autosampler
user ....

When we inject four replicates of a test sample, the signal in the firs=
t
run is 20-30% lower than in the three that follow, which are in pretty go=
od
agreement. This happens whether the replicates are obtained by sampling =
the
same vial four times, or by sampling four separate vials once each.
Moreover, a blank run follows every test run, and shows no significant
peaks, so it's hard to see how some sort of cross contamination is
occurring. =20

All insights gratefully received!

John Holt

Pasteur M=E9rieux Connaught