Crosslinking proteins to membrane

Joseph Fernandez (fernaj@rockvax.rockefeller.edu)
Tue, 17 Nov 1998 16:31:55 -0500 (EST)

80% of my protein transfers to nitrocellulose or PVDF.
90% of that is gone after 4 hours in TBS
My western blot signal sux.
Is there any way to covalently attach protein to a membrane?
I am trying glutaradehyde and baking. There has to be a better way.

Thanks,

James Bibb
c/o Joe Fernandez

Joseph Fernandez
Associate Director
The Rockefeller university
Protein/DNA Technology Center
1230 York Ave. New York, NY 10021
Phone: (212)-327-8869
FAX : (212)-327-8620
email: fernaj@rockvax.rockefeller.edu
Lab Web Page: http:\\pdtc.rockefeller.edu