Re: HPLC: organic base.
David A. Schooley (schooley@unr.edu)
Wed, 18 Nov 1998 08:59:00 -0800
Michael-
We have used 10 mM triethylammonium phosphate at 220 nm at fairly
high sensitivity without too much difficulty. Have one such chromatogram
published using Andy Alpert's polyHydroxyethyl A support (HILIC) (see PNAS
92, 12325, 1995). We use Pierce Sequanal grade Triethylamine, which is
always kept refrigerated and is SUPPOSED to be flushed with N2. It does
have appreciable absorbance at 220, and to obtain the flatest baseline you
may need to juggle the concentration of TEA in buffer A vs. B.
Use of less pure TEA, or putting TEA in either ONLY A or B, could
contribute to your problem.
David
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@med.unr.edu
tel: (702) 784-4136; fax (702) 784-1419