Chromatography and methionine

VERNON SHOUP (vernon.shoup@regpha.com)
20 Nov 98 10:30:19 -0400

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We are working with an E.coli-produced recombinant protein (21,000 MW, 3 methionines, no cysteines) that apparently has some methionine-to-norleucine variants. One of these variants chromatographs very closely to the parent protein across a number of purification techniques: cation and anion exchange, HIC, acid rpHPLC. Does anyone have any suggestions about how to specifically enhance resolution between these species, so we can quantitate the variant without doing a peptide map?

Vernon

Vernon A. Shoup
Regeneron Pharmaceuticals, Inc.
Rensselaer, NY 12144

(518)488-6012
vernon.shoup@regpha.com

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