disulphide detection

Michael Jobling (m.jobling@pgrad.unimelb.edu.au)
Mon, 23 Nov 1998 11:35:59 +1000

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Hi everybody.
We are wondering if anybody knows of a method of detecting disulphide bonds
(or the lack of) that doesn't involve HPLC or mass spec. We have a 27kDa
protein with only one disulphide present in the natural state. Is it
possible to look for a characteristic S-S absorbance by IR or UV? Is there
a chemical test for disulphides?
Any suggestions (stupid ones too, they often work best!) would be most welcome.
Regards
Michael

Michael Jobling
Dept. of Pathology
Uni. of Melbourne
m.jobling@pathology.unimelb.edu.au

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