Re: From Maria Lorenzi
Howard, Eric F (eric.f.howard@vanderbilt.edu)
Tue, 24 Nov 1998 10:24:53 -0600
In the middle of the problem now. Hopefully I will be able to solve it
today with a new valve block. I am going to assume you are using a 49X
that is at least 2 years old. There seems to be a problem with the
membranes in the delivery blocks. Have you tried the cartridge block
tests? If you find both of these tests fail the initial step then I
would block off the open port on the block that attaches to 23 and do
the test again. Also you can open valve 15 and look for bubbles in the
bottles r3, S3, s2 and s1. I found that R3 was leaking and adding air
into the lines. New block will cost $1993 (9 port solvent block part #
603454)
Eric
On Mon, 23 Nov 1998 20:57:51 +0100 Maria Lorenzi
<mlorenzi@ascu.unian.it> wrote:
> Hello!
> I have a question for you.
> I would like to know if what happen in my sequencer happens to you too.
> During the delivery of R3, S3, and S2 I can see some bubbles in the
> corrispondent tubes.
> Is it normal or there is something wrong?
> Thank you
> Maria
>
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Howard, Eric F
Vanderbilt University
Email: eric.f.howard@Vanderbilt.Edu
Protein Sequencing and Amino Acid Analysis Laboratory
615-322-6355