I assume that you mean PVDF which has had protein blotted to it and
stained. Providing that the membrane was washed well with water after
destain to remove all traces of acid, and dried thoroughly, then just keep
it clean in a sealed plastic bag. Storage at room temperature or in the
freezer is optional - I'm not sure it makes much difference. Some workers
like to take added precautions of gassing the bad with nitrogen or argon
before sealing, but I'm not sure that makes much difference either. It is
probably sensible to keep the membrane out of bright light to prevent
bleaching of the Coomassie dye and limit any potential photo-oxidation, but
this can be achieved by putting the membrane in an envelope. We have
certainly sequenced spots after >1yr at room temperature. Dryness and
acid-free seems to be the most important parameters in our experience.
Perhaps others will share their views if different.
Len
>What is the best way for long term storage of PVDF membrane ?
>
>Aki Hoji (akhst7+@pitt.edu)
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html