You don't say if you are using a 373 or 377. We genotype using 373's
and have
seen sporadic lines which became more frequent prior to the stage motor
going out. Once we had a couple of gels with lots of lines all through
the gel when the PMT was getting flakey. We also saw some VERY bizarre
gels when a lazer was overheating-- we had blank stripes across the
gel as the lazer kicked in and out. Someone else was worried that it
was related to a matrix they were trying to make, but I doubt that would
do it.
Linda
saeed wrote:
>
> Hello everyone:
>
> We are seeing red, blue & green lines across the gel. These lines are
> random throughout the gel. They started to appear all of a sudden about
> 2 days ago. It seems these lines could be due to electronic noise? Has
> anyone experience anything like it?
>
> Thank you
>
> Saeed Raees
> Ana-Gen Technologies, Inc.
> 4015 Fabian Way
> Palo Alto, CA 94303
> http://www.ana-gen.com
--Linda Wood Ballard Genomics Core Facility Huntsman Cancer Institute 7370 Eccles Institute of Human Genetics University of Utah Salt Lake City, UT 84112-5330
(801) 581-3875 FAX (801) 585-3833
--Linda Wood Ballard Genomics Core Facility Huntsman Cancer Institute 7370 Eccles Institute of Human Genetics University of Utah Salt Lake City, UT 84112-5330
(801) 581-3875 FAX (801) 585-3833