1) Do you generally use a wide mass range or a narrow one when you
utilize protein identification programs such as PeptideSearch, ProFound
and others? It is implied that one knows the apparent molecular mass
from the 2D-PAGE. What are the pros and cons for wide and narrow mass
ranges?
2) Is there any algorithm difference among those programs around
based on fragment masses for protein identification? Or are they all
using the same principles?
Thank you in advance for your comments.
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Prof. Marcelo Valle de Sousa
Brazilian Center for Protein Research and Services
Laboratory of Biochemistry and Protein Chemistry
Departament of Cell Biology
University of Brasilia
Brasilia - DF Brazil
CEP 70910-900
Tel: (061)307-2142
Fax: (061)272-4548
e-mail: mvsousa@unb.br
http://www.unb.br/cbsp
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