P2D - Making IPG strips -Reply

Henry Wolfe (hnwo@americas.nycomed.com)
Fri, 04 Dec 1998 14:33:29 -0700

My lab is bringing AAA again after some years. We will be using
AccuTag derivatization and have a peptide for which determination of
cysteines/cystines and tyrosines is important. I am interested in knowing
what the people who have been doing this work steadily find to be the
best hydrolysis protocol(s) to preserve these residues. Thanks in
Advance

Hank Wolfe
Nycomed Amersham
Wayne, Pa