Re: Digest
Deb McMillen (mcmillen@morel.uoregon.edu)
Sun, 6 Dec 1998 17:42:06 -0800 (PST)
Dear Ulf,
I have stored the GluC (from Boehringer Mannheim) at 1 ug/ul, taken up
into
distilled water, at 4 deg. I think I have used this solution for up to 1
month. =20
The other option is to take up the enzyme in water, then aliquot it into
eppendorfs and re-lyophilize and store dry again. I have kept lyophilize=
d
enzyme (straight from Boehringer) for quite a while at 4 deg and had it
still be active. Now, I have never looked closely at the activity--other
than to see that I get the cuts that I wanted in my standard proteins (an=
d
assume in my unknowns)--but I'm sure that I am never looking at 100%
cleavage of all sites.
This enzyme is definitely not as robust as endo lys C or trypsin.
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR
On Fri, 4 Dec 1998, Ulf Hellman wrote:
> Dear all,
> For some strange reason, I have always been unhappy with the stability =
of
> Staff V8 GluC protease. Trypsin (Promega) and LysC (the Achromobacter
> stuff) behave extremely well in this respect, but how do I store aliquo=
ted
> GluC protease (Promega seq grade)?
> Dissolve the 20 =B5g in what and store how?=20
> Thanks and enjoy the weekend! /Ulf
> ******************************************************
> Ulf Hellman, Ph. D. =20
> Adj. Prof.
> Ludwig Institute for Cancer Research :
> Box 595 =09
> S-751 24 Uppsala =09
> Sweden=09
> =09
> Phone +46 18 160423 =09
> Fax +46 18 160420
> E-mail ulf.hellman@licr.uu.se
> E-mail (alt.) cykeldoktorn@hotmail.com
> Lab's home page: http://www.licr.uu.se
>=20