Re: Modification of Asn

Deb McMillen (mcmillen@morel.uoregon.edu)
Wed, 9 Dec 1998 09:09:15 -0800 (PST)

Per-Ingvar,
Is this a recombinant protein? Or a native protein where there has
been a mutation? The codons for Gly (GGU and GGC) differ
from those for Asn (AAU and AAC) by only a substitution of a G for an A.

Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97405=20

On Wed, 9 Dec 1998, Per-Ingvar Ohlsson wrote:

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> Dear ABRF-er,
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> Repeated N-terminal sequence analysis of a partly known protein on a
> Prosice 494 has given glycine instead of an expected asparagin. Differ=
ent
> batches gave the same result. I wonder if someone has seen this and/or =
can
> explain the type of modification of asparagin that gives the same reten=
tion
> time as Gly.
> Thank you in advance.
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> Per-Ingvar
>=20
> Per-Ingvar Ohlsson, Dr Med Sci, Department of Medical Biochemistry and
> Biophysics, Ume=E5 University, S-907 87 Ume=E5, Sweden
> tel +46 90 786 6744, fax +46 90 136310, e-mail pio@panther.cmb.umu.s=
e
>=20
>=20