O-18 exchange

chris halkides (halkidesc@UNCWIL.EDU)
Wed, 09 Dec 1998 18:43:20 -0400

>Hello, does anyone know a commerical source for ICH2CO(18)NH2 or ICH2CO(18)OH
>(i.e., O18 labeled iodoacetamide or iodoacetic acid). Thanks.
>
>Jiang

Hello Jiang,

I don't know of a commercial supplier, but it should be possible to
exchange at low pH (0-1) in the presense of excess labeled water.
Carboxylic acids have been prepared in this manner (Van Etten? Methods in
Enzymology). Let me know if you need more precise references.

I might try using HI as the acid to minimize the chances of
displacing the iodo group, but hydrolysis (loss of iodide) might still be a
problem. I have never seen a hydrolysis rate versus pH study for these
compounds, but one may exist. I would expect that the loss of label at pH
7 is slow.

Good Luck,

Chris

weather: about a week of Indian Summer has just finished up. Highs in the
80's.

Christopher Halkides
Dept. of Chemistry, UNCW
601 S. College Road
Wilmington, NC 28403-3297
(910) 962-7427