Hope it helps
__________________________________
Brett Phinney
North Carolina State University
Department of Biochemistry
Ph# 919-515-5786, Fax 919-515-2047
ICQ# = 12442943
> -----Original Message-----
> From: Association of Biomolecular Resource Facilities
> [mailto:abrf-request@aecom.yu.edu]On Behalf Of iap@iname.com
> Sent: Friday, December 04, 1998 8:42 PM
> To: Recipients of ABRF List
> Subject: MALDI and detergents
>
>
> I checked the ABRF electronic board archives but I was unable to
> find information, although I would have thought that the topic
> must have been discussed at some point: Have people identified
> detergents which can be used effectively to do "standard" protein
> chemistry manipulations (e.g. solubilize proteins) but which are
> not detrimental to MALDI-TOF mass spectrometry with the typical
> UV lasers at 337 nm? If so, what are these detergents, at what
> levels they are more-or-less benign, what cleanup steps are
> commonly carried out prior to mass spectrometry, what types, if
> any, of columns or resins are used, etc. I guess I am looking
> for some product or methodology sufficiently different from the
> obvious, which would be, for example, use of as small an amount
> of, say, SDS and removal of as much of it as one can. I am
> particularly interested in detergents that can be removed (or
> would not intefere) in the presence of peptide and protein
> mixtures (so gel filtration or dialysis could!
> !
> not be used). Anyone with experience in this area that is
> applicable to routine use? Thanks.
>
> Ioannis Papayannopoulos
> Astra Arcus USA, Inc.
> Worcester, MA
>
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