Re: Modification of Asn

Reed Harris (reed@gene.COM)
Fri, 11 Dec 1998 09:14:27 -0800

The PTH-derivative of N-methyl-Asn (found in phycobiliproteins) has
been reported to co-elute with PTH-Ser [see Klotz and Glazer (1987) J.
Biol. Chem. 262; 17350-17355].

--
Reed Harris
Genentech, Inc.
(650) 225-4187 Phone
(650) 225-3554 FAX
reed@gene.com  E-Mail

> Dear ABRF-er, > > Repeated N-terminal sequence analysis of a partly known protein on a > Prosice 494 has given glycine instead of an expected asparagin. Different > batches gave the same result. I wonder if someone has seen this and/or can > explain the type of modification of asparagin that gives the same retention > time as Gly. > Thank you in advance. > > Per-Ingvar