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Manfred,
I have been away for Christmas and have only just read your message, and
Deb McMillen's reply. Maybe it is coincidence, but I had similar
problems during December - large peak around the position of pth-N,
somtimes big enough to obliterate half the chromatogram, but not on
every run. This is on an old (very) 470A-190A, and suspecting
contamination/other problem of lines etc, I have replaced the easier
ones, plus column, reagents, etc. The problem seems less bad, but I
still get an occasional peak of unknown, but at the pth-M position. I do
not think that this is an air bubble problem as suggested by Deb
McMillen - the odd peaks do not rise and fall that rapidly, they look
like real peaks.
Is it just coincidence that we both suffer this sort of problem at the
same time? Is everyone else okay out there in sequencerland?
Bryan Smith
Celltech Therapeutics Ltd
216 Bath Road
Slough
Berks SL1 4EN
UK
-----Original Message-----
From: Manfred Raida [SMTP:mraida@gmx.net]
Sent: 21 December 1998 02:29
To: Recipients of ABRF List
Subject: big peak in pth-chromatogram
Hallo to all,
since a few days I have a strange problem with the separation of
pth-amino
acids on my 494 Procise. In several of the chormoatograms a big
peak
starting directly after N and going up to the upper detection
limit occurs. The
peaks goes up very fast and then "elutes" over the whole
chromatogram. We
checked the pumps, the chemicals, column, detector, performance,
whatever may be
the reason. It is not every chromatogram, sometimes 10 are
without problem,
sometimes on 2-3. There is no rule when it happens, the amino
acids (if
there is enough) are on this montain and can be detetcted
clearly, but for
sensitive analysis it does not work at all. It occurs in the
standard and in the
cycles.
I am very thankful for any idea.
Yours
Manfred
*************************************************
Manfred Raida
Niedersaechsisches Institut fuer Peptid-Forschung
(IPF)
Dep. of Analytical Peptide-Chemistry
Feodor-Lynen Strasse 31
D-30625 Hannover
Germany
E-Mail Manfred_Raida@compuserve.com
Tel.: +49 511 5466 210
Fax: +49 511 5466 102
**************************************************
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