Chris Clogston
cclogsto@amgen.com
Amgen, Inc.
Thousand Oaks, CA 91320-1789
> ----------
> From: Bryan Smith[SMTP:bsmith@celltech.co.uk]
> Sent: Tuesday, January 05, 1999 12:38 AM
> To: Recipients of ABRF List
> Subject: Big peaks in pth chromatogram
>
> Manfred,
> I have been away for Christmas and have only just read your message, and
> Deb McMillen's reply. Maybe it is coincidence, but I had similar problems
> during December - large peak around the position of pth-N, somtimes big
> enough to obliterate half the chromatogram, but not on every run. This is
> on an old (very) 470A-190A, and suspecting contamination/other problem of
> lines etc, I have replaced the easier ones, plus column, reagents, etc.
> The problem seems less bad, but I still get an occasional peak of unknown,
> but at the pth-M position. I do not think that this is an air bubble
> problem as suggested by Deb McMillen - the odd peaks do not rise and fall
> that rapidly, they look like real peaks.
>
> Is it just coincidence that we both suffer this sort of problem at the
> same time? Is everyone else okay out there in sequencerland?
>
> Bryan Smith
> Celltech Therapeutics Ltd
> 216 Bath Road
> Slough
> Berks SL1 4EN
> UK
>
> -----Original Message-----
> From: Manfred Raida [SMTP:mraida@gmx.net]
> Sent: 21 December 1998 02:29
> To: Recipients of ABRF List
> Subject: big peak in pth-chromatogram
>
> Hallo to all,
> since a few days I have a strange problem with the separation of
> pth-amino
> acids on my 494 Procise. In several of the chormoatograms a big peak
> starting directly after N and going up to the upper detection limit
> occurs. The
> peaks goes up very fast and then "elutes" over the whole
> chromatogram. We
> checked the pumps, the chemicals, column, detector, performance,
> whatever may be
> the reason. It is not every chromatogram, sometimes 10 are without
> problem,
> sometimes on 2-3. There is no rule when it happens, the amino acids
> (if
> there is enough) are on this montain and can be detetcted clearly,
> but for
> sensitive analysis it does not work at all. It occurs in the
> standard and in the
> cycles.
> I am very thankful for any idea.
> Yours
> Manfred
> *************************************************
> Manfred Raida
> Niedersaechsisches Institut fuer Peptid-Forschung
> (IPF)
> Dep. of Analytical Peptide-Chemistry
> Feodor-Lynen Strasse 31
> D-30625 Hannover
> Germany
> E-Mail Manfred_Raida@compuserve.com
> Tel.: +49 511 5466 210
> Fax: +49 511 5466 102
> **************************************************
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