Peptide Synthesis: Azido Linkage

Kenneth Williams (Kenneth.Williams@yale.edu)
Wed, 06 Jan 1999 08:59:02 -0500

I am looking for an easy means to add an azido functional group to either
end of a short (8-mer) peptide composed of glycine and arginine that will
be used to help localize the active site of an enzyme. The peptide will be
synthesized via Fmoc chemistry and a short linker can be added to either
end. The best idea I have come up with so far is to react the free
N-terminus of the cleaved peptide with a reagent like
N-Hydroxysuccinidyl-4-azidosalicylic acid - which would also permit the
facile I125 labelling of the peptide. Are there any other suggestions or
does anyone see a problem with this approach.

Thanks very much and best wishes for the New Year,

Ken Williams