Protein Deglycosylation

John Nechtman (JNECHTMA@MAIL.MCG.EDU)
Wed, 06 Jan 1999 16:13:00 -0500

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We need two procedure to deglycosylate an immunoprecipitated nuclear protein that binds wheat germ agglutinin(WGA):
1) before separation by SDS-PAGE
2) after separation and transfer to PVDF membrane

John Nechtman
Medical College of Georgia
Molecular Biology Core Facility
Dept Biochemistry Molecular Biology

Next message: Yalin Wei: "formamide gels"
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