Re: Re; MET sulfoxide

Rod Levine (rlevine@nih.gov)
Fri, 08 Jan 1999 17:24:42 -0500

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At 02:49 PM 1/8/99 -0500, Joseph Fernandez wrote:
> Here's a question. Has anyone observed strange peask that could be
>Met-Sulfoxide on the HP sequencer? I believe they do not use DTT in the R4
>but I cannot recall if I have sequenced a peptide containing oxidized MET
>(based on MALDI-TOF) on our instrument. Thanks
>
Joe,

In our hands Met-SO is generally reduced back to Met on the HP sequencer. See,
eg,
Methionine residues as endogenous antioxidants in proteins.
Proc.Natl.Acad.Sci.U.S.A. 93:15036-15040, 1996.

Rod

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