Met-Thr bonds are resistant to CNBr cleavage, so this will contribute to
incomplete cleavage patterns, depending on the frequency of occurrence in
the sequence.
Len
>Rod, I was wondering if there are mets that are so buried or in a
>microenvironment (I love that term) that CNBr cannot attack them--even
>though they are met and not met sulfoxide. I've always understood that
>CNBr maps of proteins were most likely not going to be complete--but is
>that only dependent on the presence of met sulfoxide sites?
>Thanks,
>Deb McMillen
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html