Re: Protein sequencing: retention time of oxidized methionine

Carol Beach (cmbeach@pop.uky.edu)
Mon, 11 Jan 1999 08:31:41 -0500 (EST)

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At 09:59 AM 1/11/99 +0000, Len Packman wrote:
>Deb
>
>Met-Thr bonds are resistant to CNBr cleavage, so this will contribute to
>incomplete cleavage patterns, depending on the frequency of occurrence in
>the sequence.
>
>Len
>

Deb:
Want to read more about this? Check out "Enhancement of Cyanogen
Bromide Cleavage Yields for Methionyl-Serine and Methionyl-Threonine Peptide
Bonds" by Raymond Kaiser and Lorraine Metzka in Analytical Biochemistry 266,
1-8 (1999). The article includes nifty chemical reaction schemes.

Carol

Carol Manley Beach, Ph.D.
Protein Sequence Analysis
Macromolecular Structure Analysis Facility
232 Combs Cancer Research Building
University of Kentucky
Lexington, KY 40536-0096

cmbeach@pop.uky.edu
(606)257-4932 (office)
(606)257-4475 (lab)
(606)323-3909 (FAX)

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