Re: 2D gels => Digest 15/01/99

Tony.Houthaeve (Tony.Houthaeve@rug.ac.be)
Fri, 15 Jan 1999 11:27:33 +0100 (MET)

>So, what is the problem you ask? While
>cutting into wee tiny pieces, the pieces: 1.) fly all over, 2.) stick to
>the razor blade...in addition, the human doing the cutting goes blind, gets a
>big headache and contaminates the sample with hair and saliva (from muttering
>disgustedly). Any ideas??

Dear Jim,

there are several things what could help.

If the pieces fly all over => try cut the pieces in a small drop (20ul) of water
In addition to sticking and flying around => use an ionizing air blower or a static ionizing bar to reduce all charging of plastic and other stuff (can get it from SIMCO in Hatfield USA or others I guess)
Big headaches => try cutting them under an illuminated magnifier
Hair and other things => try working as much as possible in a laminar flow or why even not work in an Class 10.000 clean room; beside of the traditional wear gloves all the time and buy only highly purified chemicals.

I guess you'll easily reach below 1 pmol then.

Hope this info is of any use.

Regards,

Tony H.

===================================================
Dr. Ing. Houthaeve Tony
VIB
Dept. Med. Prot. Research
University Gent
K.L. Ledeganckstraat 35
B-9000 Gent
Belgium

tel. (32) - 9 - 264 52 91
fax. (32) - 9 - 264 52 93

Tony.Houthaeve@RUG.ac.be
http://allserv.rug.ac.be/~thouthae/
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