since some time we have a problem with our Prism 377 DNA sequencer. Since
our upper buffer reservoir is slightly leaking, we have to control the
buffer level in the evening. Until December everything was o.k., but when
we made an upgrade from 48 to 96 lanes, at the beginning we got a line
which started at baseline level and then turned into a red stripe until the
end of the scan line. This line occured at the point when we opened the
door. Since approximately two weeks now, the red stripe turned into a line
with signal intensity at all for colors for only one scan line as well.
At first we thought it could be the laser, but it seems to be o.k.
Has anybody of you already also encountered this problem or knows something
about its origin? Is there eventually a connection to the "corrupted gel
file" topic discussed some time ago which I unfortunately did not pursue
(because I wasn't involved in DNA sequencing at this time ...:-(... )? Any
help would be appreciated. Thanks in advance.
Yours sincerely,
Marcus
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Marcus Macht
ZMMK-Servicelabor
Joseph-Stelzmann-Str. 52
50931 Koeln
Tel.: +49 221 478-6995
Fax: +49 221 478-6977
e-mail:Marcus.Macht@uni-koeln.de
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