AAA hydrolysis

cameron.olbert@arcb.us.astra.com
Wed, 20 Jan 1999 15:32:58 +0100

Dear ABRF members,
We are currently trying to do AAA on a protein in a 10mM Tris 1%
n-Octyl-Glucopyranoside buffer and at the end of the hydrolysis step we are
seeing a black mass coating the bottom of the sample tubes. We are running
a vapor phase hydrolysis with 6N HCl 1% phenol, at 110C for 20 hours,
purging the reaction vial with nitrogen before hydrolysis. We believe the
black mass is caused by the nOG in the buffer (we've seen it in a buffer
blank). Any suggestions on how to avoid this hydrolysis problem are greatly
appreciated.

Thank you in advance
Cameron Olbert
Astra Research Center Boston
cameron.olbert@arcb.us.astra.com