Sheryl Christofferson
OMRF Sequencing Facility
Marcus Macht wrote:
> Dear colleagues,
>
> since some time we have a problem with our Prism 377 DNA sequencer. Since
> our upper buffer reservoir is slightly leaking, we have to control the
> buffer level in the evening. Until December everything was o.k., but when
> we made an upgrade from 48 to 96 lanes, at the beginning we got a line
> which started at baseline level and then turned into a red stripe until the
> end of the scan line. This line occured at the point when we opened the
> door. Since approximately two weeks now, the red stripe turned into a line
> with signal intensity at all for colors for only one scan line as well.
> At first we thought it could be the laser, but it seems to be o.k.
> Has anybody of you already also encountered this problem or knows something
> about its origin? Is there eventually a connection to the "corrupted gel
> file" topic discussed some time ago which I unfortunately did not pursue
> (because I wasn't involved in DNA sequencing at this time ...:-(... )? Any
> help would be appreciated. Thanks in advance.
>
> Yours sincerely,
> Marcus
> ****************************************************************************
> ********
> Marcus Macht
> ZMMK-Servicelabor
> Joseph-Stelzmann-Str. 52
> 50931 Koeln
> Tel.: +49 221 478-6995
> Fax: +49 221 478-6977
> e-mail:Marcus.Macht@uni-koeln.de
> ****************************************************************************
> ********