I have a suggestion for you but I don't know it will work or not. You can
use Fmoc-Cys(acm)-OH (ANASpec) to protect the Cys. Use MBS to couple the
peptide to KLH and than get rid of the acm. One thing you should know is
that you peptide may form inter molecular disulfide bond and this may
prevent the antibody production. We do not have mush lock on a peptide
which has Cys.
Best Regards,
Bob Lee,
Head of the Protein Research Lab. at UIC
On Wed, 20 Jan 1999, Michael Jobling wrote:
> Hi everybody.
> We are trying to conjugate KLH to a peptide (via a free cysteine). The
> problem is that our peptide has two cysteine residues which form a
> disulphide bond quite readily. After reduction by DTT, the disulphide
> reforms during dialysis to remove the DTT from the reaction mixture. Apart
> from running a column or 'quicker' method of removing the DTT, so the
> disulphide doesn't have a chance to reform, does anybody have a suggestion
> as to how we might be able to maintain a free S-H to add the KLH to.
> Thanks in advance
>
>
> Michael Jobling
> Dept. of Pathology
> Uni. of Melbourne
> m.jobling@pathology.unimelb.edu.au
>
>