line in gel file
Loc-Ha Le (lle@bio101.com)
Thu, 21 Jan 1999 16:32:40 -0800
>Date: Thu, 21 Jan 1999 15:40:45 -0800
>To: Marcus.Macht@uni-koeln.de
>From: Loc-Ha Le <lle@bio101.com>
>Subject: Troubleshooting
>Cc:
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>>Date: Thu, 21 Jan 1999 15:27:34 -0800
>>To: :Marcus.Macht@uni-koeln.de
>>From: Loc-Ha Le <lle@bio101.com>
>>Subject:
>>Cc:
>>Bcc:
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>> Dear colleagues,
>>>
>>> since some time we have a problem with our Prism 377 DNA sequencer. Since
>>> our upper buffer reservoir is slightly leaking, we have to control the
>>> buffer level in the evening. Until December everything was o.k., but when
>>> we made an upgrade from 48 to 96 lanes, at the beginning we got a line
>>> which started at baseline level and then turned into a red stripe until the
>>> end of the scan line. This line occured at the point when we opened the
>>> door. Since approximately two weeks now, the red stripe turned into a line
>>> with signal intensity at all for colors for only one scan line as well.
>>> At first we thought it could be the laser, but it seems to be o.k.
>>> Has anybody of you already also encountered this problem or knows something
>>> about its origin? Is there eventually a connection to the "corrupted gel
>>> file" topic discussed some time ago which I unfortunately did not pursue
>>> (because I wasn't involved in DNA sequencing at this time ...:-(... )? Any
>>> help would be appreciated. Thanks in advance.
>>>
>>> Yours sincerely,
>>> Marcus
>> Marcus Macht
>> ZMMK-Servicelabor
>> Joseph-Stelzmann-Str. 52
>> 50931 Koeln
>> Tel.: +49 221 478-6995
>>Fax: +49 221 478-6977
>>
>>
>Hi Marcus,
>>
>>The leak from upper chamber can be stop by changing the new gasket ( with
>>some grease). Make sure the interface between plate and gasket is dried.
>>If it is wet , it may conduct the leak slowly. Of course do not not fill
>>buffer too full may also cause that problem (low buffer may cause the
>>stop of the run too!) . The line occurred at the point when you open the
>>door may cause from 2 reasons.
>>
>> 1/. The plate may get some fluorescent contamination. Soak
>>in the Multi-Terge( Biodegradable Detergent and Radioactive
>>Decontaminant) or clean by polishing the plate in a circular motiion
>>with Cerium Oxide (15g in 50ml water)
>>( Rhodite 90) of Universal Photonics Inc. then rinse with hot tap water.
>>
>> 2/. Check the voltage, current and power when the machine
>>start ( also check the voltage during the run by looking at the
>>condition button beside other electrophoregram button) , compare with the
>>other machine. If it is not right , change the voltage. Sometime
>>because the 10XTBE in your gel mix and buffer are not the same, then
>>between the gel and buffer it has a competition of fluorescent ions may
>>give you something " bizzarre". Good luck and let me know if how it
>>works.
>>
>>
>>
>>
>>Loc-Ha Le,Scientist .
>>BIO101 Inc.
>>
>>
>>
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